Search results for " metabarcoding analyses"

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Molecular analysis ofPhytophthoradiversity in nursery-grown ornamental and fruit plants

2015

The genetic diversity of Phytophthora spp. was investigated in potted ornamental and fruit tree species. A metabarcoding approach was used, based on a semi-nested PCR with Phytophthora genus-specific primers targeting the ITS1 region of the rDNA. More than 50 ITS1 sequence types representing at least 15 distinct Phytophthora taxa were detected. Nine had ITS sequences that grouped them in defined taxonomic groups (P. nicotianae, P. citrophthora, P. meadii, P. taxon Pgchlamydo, P. cinnamomi, P. parvispora, P. cambivora, P. niederhauserii and P. lateralis) whereas three phylotypes were associated to two or more taxa (P. citricola taxon E or III; P. pseudosyringae, P. ilicis or P. nemorosa; and…

Phylotypegenus-specific primers metabarcoding analyses nurseries Phytophthora spp. potted plantsGenetic diversitySettore AGR/12 - Patologia Vegetalegenus-specific primersPlant ScienceHorticultureBiologybiology.organism_classificationTaxonIntergenic regionnurseriesBotanyOrnamental plantGeneticspotted plantsTaxonomic rankPhytophthoragenus-specific primers; metabarcoding analyses; nurseries; Phytophthora spp.; potted plantsmetabarcoding analysesAgronomy and Crop SciencePhytophthora sppFruit treePlant Pathology
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